Antibody Dilution Calculator

What is Antibody Dilution Calculator?

An antibody dilution calculator is a tool that helps researchers determine the optimal dilution ratios for primary and secondary antibodies in various immunoassays. It calculates the exact volumes of antibody stock and diluent needed to achieve the desired working concentration for different applications like Western blotting, ELISA, immunofluorescence, and flow cytometry.

How to Use This Antibody Dilution Calculator

Calculate optimal antibody dilutions in three simple steps:

1. Enter your stock antibody concentration and select the unit
2. Input the desired working concentration and final volume
3. Choose your application type to get recommended ranges and step-by-step protocol

When to Use

This calculator is essential when you need to:

• Prepare antibody dilutions for Western blot analysis
• Set up ELISA assays with proper antibody concentrations
• Optimize immunofluorescence staining protocols
• Calculate dilutions for immunohistochemistry experiments
• Prepare antibodies for flow cytometry analysis

Application Concentration Ranges

Typical working concentrations:

• Western Blot: 0.1-2 μg/mL
• ELISA: 0.5-5 μg/mL
• Immunofluorescence: 1-10 μg/mL
• IHC: 5-20 μg/mL
• Flow Cytometry: 0.5-5 μg/mL

Example Calculation

Stock: 1 mg/mL, Working: 2 μg/mL, Volume: 1 mL

Perfect for Western blot primary antibody.

FAQ

Q: How do I handle serial dilutions?
A: For high dilution factors (>100), the calculator provides step-by-step serial dilution protocols.

Q: What diluent should I use?
A: Common diluents include PBS with BSA, blocking buffer, or manufacturer-recommended diluents.