Bradford Assay Calculator

Calculate protein concentrations using Bradford assay standard curve analysis with linear regression and quality metrics

Bradford Assay Analysis

What is Bradford Assay Calculator?

A Bradford assay calculator that determines protein concentrations using BSA standard curves and linear regression analysis. Essential for protein quantification in biochemistry and molecular biology research.

How to Use This Bradford Calculator

Quick analysis in 3 steps:

  1. Enter BSA standards (concentration, absorbance) and sample data
  2. View automatic standard curve generation and R² value
  3. Get sample concentrations with dilution factor corrections

When to Use

This Bradford assay calculator is useful for:

  • Protein concentration determination in research
  • Quality control of protein purification
  • Standardizing protein samples for experiments
  • Verifying Bradford assay standard curve quality

Example Input

BSA standards and sample format:

Standard: 0.0, 0.120 Standard: 0.5, 0.425 Standard: 1.0, 0.735 Sample: Protein1, 0.625, 1.0

Include blank (0.0 mg/mL) for automatic correction.

Example Output

Analysis results include:

Standard Curve: y = 0.723x + 0.115 R² = 0.998 (Excellent fit) Protein1: 0.71 mg/mL (original: 0.71 mg/mL)

Includes curve equation, R² value, and concentrations.

FAQ

Q: What R² value indicates a good standard curve?
A: R² ≥ 0.95 is good, ≥ 0.98 is excellent for Bradford assays.

Q: How are dilution factors handled?
A: Final concentrations are automatically multiplied by dilution factors.