Cell Viability Calculator

Calculate cell viability for Trypan Blue, MTT/MTS, Alamar Blue, and LDH assays with IC50 analysis

IC50 Calculation (Optional)

What is Cell Viability Calculator?

A comprehensive tool for calculating cell viability across multiple assay types including Trypan Blue exclusion, MTT/MTS colorimetric assays, Alamar Blue fluorometric assays, and LDH release cytotoxicity assays.

How to Use This Calculator

Quick guide to get results:

  1. Select your assay type from the tabs
  2. Enter your experimental values
  3. Results calculate automatically
  4. Optional: Add dose-response data for IC50

When to Use

This calculator is useful when you need to:

  • Assess cell viability after drug treatment
  • Calculate cytotoxicity percentages
  • Determine IC50 values from dose-response curves
  • Compare different treatment conditions

Example Input - Trypan Blue

Sample data for Trypan Blue counting:

Live cells: 150 Dead cells: 25 Dilution factor: 2 Volume counted: 10 μL

Results show viability % and cell concentration.

Example Output

Expected results format:

Cell Viability: 85.7% Cell Concentration: 3.5 × 10⁵ cells/mL Cytotoxicity: 14.3%

Results can be copied for documentation.

FAQ

Q: Which assay type should I use?
A: Trypan Blue for direct cell counting, MTT for metabolic activity, Alamar Blue for non-destructive assays, LDH for cytotoxicity.

Q: How is IC50 calculated?
A: Using log-linear interpolation from dose-response curve data.