Calculate Colony Forming Units per mL from bacterial culture plate counts and dilution factors with statistical validation
| Plate | Colony Count | Dilution Factor | Volume (µL) | CFU/mL | Log10 CFU/mL | Status |
|---|
A Colony Forming Units (CFU) calculator is an essential microbiology tool that determines the concentration of viable bacterial cells in a culture. It calculates CFU/mL from colony counts on agar plates, accounting for dilution factors and plated volumes to provide accurate bacterial concentration measurements.
Calculate bacterial concentration in three simple steps:
This CFU calculator is essential for:
Sample bacterial culture data:
Plate 1: 156 colonies, 10^-5 dilution, 100 µL
Plate 2: 142 colonies, 10^-5 dilution, 100 µL
Plate 3: 38 colonies, 10^-6 dilution, 100 µLTry with your own plate counts or use the Example button.
CFU analysis results include:
Average CFU/mL: 1.45 × 10^8
Log10 CFU/mL: 8.16
95% CI: 1.32 × 10^8 - 1.58 × 10^8
Valid plates: 3/3Results include statistical validation and confidence intervals.
Q: What is the optimal colony count range?
A: 30-300 colonies per plate for accurate counting and statistical validity.
Q: How are confidence intervals calculated?
A: Using t-distribution for small samples or normal distribution for large samples (n≥30).
Q: What dilution factors should I use?
A: Start with 10^-3 to 10^-7 for most bacterial cultures, adjust based on expected density.