Concatenate Genes

Join multiple gene sequences with customizable spacers for synthetic biology and operon design.

Spacer Sequence Options
Concatenation Preview (First 200bp)

What is Concatenate Genes?

This tool joins multiple gene sequences with optional spacer sequences. Essential for designing synthetic operons, fusion proteins, and multi-gene expression cassettes for cloning and synthetic biology.

How to Use This Tool

  1. Paste gene sequences in FASTA format
  2. Select a spacer type or enter custom
  3. Preview the concatenation structure
  4. Download the joined sequence

When to Use

  • Creating synthetic operons
  • Designing fusion genes
  • Building multi-gene cassettes
  • Preparing sequences for Gibson assembly

Spacer Options

Flexible linker: GGGGS for protein fusions

RBS: AGGAGG ribosome binding site

Stop codon: TAA termination signal

BamHI: GGATCC restriction site

Example Output

>Concatenated_3_genes
ATGGCTAGC[spacer]GCTAGCTA[spacer]TTAATTAA

Genes joined with selected spacers.

FAQ

Q: Can I use amino acid sequences?
A: Yes, works with DNA, RNA, or protein sequences.

Q: What's the maximum number of genes?
A: No limit - concatenate as many sequences as needed.