Dilution Calculator

Calculate dilutions using C1V1=C2V2 formula, serial dilutions, dilution factors, and stock solution preparations.

C₁ × V₁ = C₂ × V₂

What is Dilution?

Dilution is the process of reducing the concentration of a solution by adding solvent. The C1V1=C2V2 formula ensures the amount of solute remains constant.

How to Use This Calculator

  1. Choose dilution type (simple, serial, or factor)
  2. Enter known values
  3. Select what to solve for (simple dilution)
  4. Get instant results with instructions

When to Use

  • Preparing working solutions from stock
  • Setting up standard curves
  • Serial dilutions for titrations
  • Cell culture media preparation

Common Dilutions

1:10 = 1 part + 9 parts solvent

1:100 = 1 part + 99 parts solvent

1:2 = 1 part + 1 part solvent

1:5 = 1 part + 4 parts solvent

Tips & Tricks

  • Always add solute to solvent, not vice versa
  • Mix thoroughly after each dilution
  • Use fresh pipette tips between dilutions
  • Label all tubes clearly

FAQ

Q: What's the difference between dilution factor and dilution ratio?
A: Factor is total parts (e.g., 10), ratio shows parts separately (e.g., 1:9).

Q: How do I prepare a 1:1000 dilution?
A: Add 1 µL to 999 µL, or do serial dilutions (1:10 three times).