DNA Melting Curve Plotter

Visualize DNA melting temperature (Tm) across your sequence. Identify stable and unstable regions for PCR optimization and primer design.

Melting Curve Analysis

0
Sequence Length
0°C
Average Tm
0°C
Min Tm
0°C
Max Tm

Melting Temperature Profile

Tm Profile
GC-Rich (>60%)
AT-Rich (>60%)

Detailed Tm Data

Position (bp)Tm (°C)GC Content

What is a Melting Curve?

A DNA melting curve shows the melting temperature (Tm) across a sequence. Higher Tm indicates more stable regions (typically GC-rich), while lower Tm shows less stable regions (AT-rich). Essential for PCR and primer design.

How to Use This Tool

Quick guide to plot melting curves:

  1. Paste your DNA sequence
  2. Select window size (20 bp recommended)
  3. Click "Plot Curve"
  4. Analyze Tm profile and regions

When to Use

This tool is useful for:

  • PCR primer design and optimization
  • Identifying stable/unstable DNA regions
  • Analyzing GC content distribution
  • Predicting PCR amplification issues
  • Optimizing annealing temperatures

Understanding Window Size

Window size determines the resolution of your analysis:

  • Small (10-15 bp): High resolution, more detail
  • Medium (20-30 bp): Balanced, recommended
  • Large (40-50 bp): Smooth curve, general trends

Interpreting Results

Key metrics explained:

  • Average Tm: Overall sequence stability
  • Min/Max Tm: Range of thermal stability
  • Green regions: GC-rich, high stability
  • Orange regions: AT-rich, low stability

FAQ

Q: What's a good Tm range for primers?
A: 50-65°C is typical, with similar Tm for primer pairs.

Q: Why do I see spikes in the curve?
A: Local GC-rich regions create higher Tm values.