Visualize Gumbel distribution for alignment scores and understand P-values in BLAST
The Extreme Value Distribution (Gumbel) describes the distribution of maximum values from random samples. In BLAST, optimal alignment scores follow this distribution, not a normal distribution, allowing accurate P-value and E-value calculations.
Explore EVD parameters and their effects:
This tool is useful when you need to:
Typical BLOSUM62 parameters:
λ = 0.267, K = 0.041
Query: 500 aa
Database: 1M residues
Score: 50E-value ≈ 0.006 (highly significant)
Statistical measures:
E-value: 0.006
P-value: 0.006
Bit Score: 42.8
Significance: p < 0.01Score unlikely by chance alone.
Q: Why not use normal distribution?
A: Maximum scores from many comparisons follow EVD, which has a longer tail than normal distribution.
Q: What is characteristic value u?
A: The score where E-value = 1, calculated as u = ln(Kmn)/λ.