Remove duplicate sequences from FASTA files by sequence content or headers. Clean your sequence databases efficiently.
This tool removes duplicate sequences from FASTA files based on either sequence content or header IDs. Essential for cleaning sequence databases, removing redundancy, and preparing unique datasets for analysis.
By Sequence: Removes identical sequences regardless of headers
By Header: Removes duplicate IDs keeping unique sequences
Case Sensitive: Treats ATCG differently from atcg
>Gene1
ATCGATCG
>Gene2_duplicate
ATCGATCG
>Gene3
GCTAGCTAResults in 2 unique sequences.
Q: Which duplicate is kept?
A: By default, the first occurrence. Uncheck "Keep first" for last.
Q: Does it preserve FASTA formatting?
A: Yes, headers and sequences are maintained.