FASTA to FASTQ

What is FASTA to FASTQ Converter?

This tool converts DNA or RNA sequences from FASTA format to FASTQ format by adding quality scores. FASTQ format is required for many NGS analysis pipelines and includes base quality information alongside sequence data.

How to Use This Tool

Quick guide to convert:

1. Paste your FASTA sequences
2. Adjust quality score (default: 40 = high quality)
3. FASTQ output appears automatically
4. Download or copy for downstream analysis

When to Use

This tool is useful when you need to:

• Convert reference sequences to FASTQ format
• Create mock FASTQ files for testing pipelines
• Add quality scores to sequences lacking them
• Prepare sequences for tools requiring FASTQ input

Example Input

Sample FASTA format:

Supports single or multiple sequences.

Example Output

Expected FASTQ format:

Quality 'I' = Phred score 40 (very high quality).

FAQ

Q: What quality score should I use?
A: Score 40 (character 'I') represents 99.99% base call accuracy, suitable for most applications.

Q: Can I process multiple sequences?
A: Yes, all sequences in your FASTA file will be converted with the same quality score.