Linear DNA µg to pmol of Ends

Convert micrograms of linear DNA to picomoles of ends. Essential for ligation calculations, end-labeling experiments, and molecular cloning.

What is Linear DNA µg to pmol of Ends?

This calculator converts the mass of linear DNA (in micrograms) to the number of picomoles of DNA ends. Since linear DNA has two ends, this is essential for calculating the correct amounts needed for ligation reactions and end-labeling experiments.

How to Use This Calculator

  1. Enter DNA amount in micrograms (µg)
  2. Enter DNA length in base pairs (bp)
  3. Optionally enter volume for concentration
  4. Results appear automatically

When to Use

This calculator is useful when you need to:

  • Calculate insert:vector ratios for ligation reactions
  • Determine DNA end concentrations for kinase reactions
  • Plan end-labeling experiments
  • Optimize molecular cloning protocols

Example Calculation

Example input:

DNA Amount: 1 µg
DNA Length: 5000 bp
Volume: 20 µL

Result: 615.38 pmol of ends (30.77 pmol/µL)

Formula

The calculation uses this formula:

pmol of ends = (µg × 2 × 10⁶) / (bp × 650)

Where 650 g/mol is the average molecular weight of a DNA base pair, and 2 accounts for the two ends of linear DNA.

FAQ

Q: Why multiply by 2?
A: Linear DNA molecules have two ends (5' and 3'), so we multiply by 2 to get the total picomoles of ends.

Q: What's the difference from total pmol of DNA?
A: This calculates pmol of ends (which is 2× the pmol of DNA molecules), useful for reactions that depend on end concentration like ligation.