mRNA Calculator

Calculate extinction coefficient and concentration for mRNA with modified nucleotides including Ψ, m1Ψ, and m5C.

Measurement Parameters

mRNA Modifications

Standard uridine (U)
Replace U with Ψ (pseudouridine)
Replace U with m1Ψ (N1-methylpseudouridine)
Replace C with m5C (5-methylcytidine)

mRNA Cap Structure

What is mRNA Calculator?

This tool calculates the extinction coefficient (ε260) and concentration for mRNA molecules with modified nucleotides including pseudouridine (Ψ), N1-methylpseudouridine (m1Ψ), and 5-methylcytidine (m5C).

How to Use This mRNA Calculator

Quick guide to get results:

  1. Enter or paste your RNA sequence
  2. Select any modifications (Ψ, m1Ψ, m5C)
  3. Enter A260 absorbance if available
  4. View extinction coefficient and concentration
  5. Download or copy results

When to Use

This tool is useful when you need to:

  • Quantify mRNA vaccine concentrations
  • Calculate extinction coefficients for modified RNA
  • Determine RNA concentration from A260 measurements
  • Analyze therapeutic RNA preparations

Example Input

Sample mRNA sequence:

AUGGUUCGUGUUCCUGGUGCUGCUGCCUCUGGUGUCCAGCC...

With A260 = 1.234, Path = 1 cm, Volume = 100 μL

Example Output

Expected output format:

Molecular Weight: 163245.32 g/mol
Extinction Coefficient: 4823.45 mM⁻¹cm⁻¹
Concentration: 255.78 nM

Full analysis with nucleotide composition.

FAQ

Q: What units are used?
A: Extinction coefficient in mM⁻¹cm⁻¹, concentration in nM or ng/μL

Q: Can I analyze vaccine mRNA?
A: Yes, this tool is optimized for modified mRNA including vaccine sequences