Nucleic Acid Weight-Molar Converter

Convert between nucleic acid weight and molar quantities for dsDNA, ssDNA, RNA, and oligonucleotides. Calculate molarity from mass using sequence length.

What is Nucleic Acid Weight-Molar Converter?

This converter tool allows you to convert between weight (mass) and molar quantities of nucleic acids. It supports different types of nucleic acids including double-stranded DNA, single-stranded DNA, RNA, and oligonucleotides, each with their specific molecular weight calculations.

How to Use This Converter

Quick guide to convert nucleic acid quantities:

  1. Select your nucleic acid type (dsDNA, ssDNA, RNA, or oligo)
  2. Enter the sequence length in bases or kilobases
  3. Choose conversion direction (weight to molar or vice versa)
  4. Enter your input value with appropriate units
  5. View the calculated result instantly

When to Use

This tool is useful when you need to:

  • Calculate how many moles are in a given mass of DNA/RNA
  • Determine the mass needed for a specific molar amount
  • Prepare solutions with precise nucleic acid concentrations
  • Convert between different unit scales (ng to pmol, µg to nmol, etc.)

Example Calculation

Converting 10 µg of a 500 bp dsDNA fragment:

10 µg × (1 mol / 330,000 g) × (1 / 500 bp) = 60.6 pmol

Try the Example button for a demo calculation.

Molecular Weights Used

Average molecular weights per base:

  • dsDNA: 660 g/mol per bp
  • ssDNA: 330 g/mol per base
  • RNA: 340 g/mol per base
  • Oligo: 330 g/mol per base (ssDNA)

FAQ

Q: What's the difference between dsDNA and ssDNA calculations?
A: dsDNA has two strands, so its molecular weight per base pair is approximately double that of ssDNA.

Q: Can I convert concentrations (M, mM, µM)?
A: Yes, when converting molar to weight, you can select concentration units. The tool assumes 1L volume for concentration calculations.