Protein GRAVY Calculator

Calculate Grand Average of Hydropathy (GRAVY) values using the Kyte-Doolittle hydropathy scale. Analyze protein hydrophobicity for structure and membrane association prediction.

Decimal Precision:
3

What is Protein GRAVY?

GRAVY (Grand Average of Hydropathy) is a numerical value representing the overall hydrophobicity or hydrophilicity of a protein. It's calculated by adding the hydropathy values for each amino acid residue (using the Kyte-Doolittle scale) and dividing by the sequence length. Positive GRAVY values indicate hydrophobic proteins, while negative values indicate hydrophilic proteins.

How to Use This Calculator

Quick guide to calculate GRAVY:

  1. Paste your protein sequence (plain text or FASTA format)
  2. GRAVY values are calculated automatically as you type
  3. Download or copy results for analysis

When to Use GRAVY

This calculator is useful when you need to:

  • Predict protein membrane association or localization
  • Assess protein solubility and aggregation potential
  • Characterize protein surface properties for structural analysis
  • Compare hydrophobicity between protein sequences

Example Input

Sample protein sequence (cytochrome c):

MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE

Try with your own sequence or use the Example button.

Example Output

Expected output format:

Sequence 1: -0.492
(Hydrophilic protein)

Positive values = hydrophobic, Negative values = hydrophilic.

FAQ

Q: What does a positive GRAVY value mean?
A: Positive values indicate hydrophobic proteins, likely to be membrane-associated or buried in protein cores.

Q: Can I analyze multiple sequences at once?
A: Yes, input multiple sequences in FASTA format and get individual GRAVY values for each.