Restriction Digest Analyzer

Perform virtual restriction enzyme digests on DNA sequences. Analyze cut sites, predict fragment sizes, and plan cloning experiments.

Restriction Enzymes & Settings
Primary Enzyme
Secondary Enzyme (Optional)
Tertiary Enzyme (Optional)
Molecule Type
Linear Circular
Fragment Map & Analysis

Cut Sites & Fragments

Red lines show cut sites, colored blocks represent resulting fragments

Fragment #Size (bp)StartEndCut bySequence

What is Restriction Digest?

Restriction digest analysis predicts how restriction enzymes will cut DNA sequences. Essential for cloning, mapping, and molecular biology experiments planning.

How to Use This Tool

Analyze restriction digests in 4 steps:

  1. Enter your DNA sequence
  2. Select restriction enzymes
  3. Choose linear or circular DNA
  4. View cut sites and fragments

When to Use

This tool is essential for:

  • Cloning strategy planning
  • Vector linearization
  • Fragment size prediction
  • Gel electrophoresis planning

Example Input

Sample plasmid DNA sequence:

GAATTCAAGCTTGGATCCGCATGC...

Try with EcoRI, HindIII, BamHI enzymes

Example Output

Fragment analysis results:

Fragment 1: 1,234 bp (1-1234)
Fragment 2: 567 bp (1235-1801)
Cut by: EcoRI, HindIII

FAQ

Q: How many enzymes can I use?
A: Up to 3 enzymes simultaneously for complex digests.

Q: What's the difference between linear and circular?
A: Circular DNA (like plasmids) has no free ends, affecting fragment analysis.