Restriction Map

Map restriction enzyme cut sites on DNA sequences. Visualize enzyme positions for molecular cloning and digestion planning

Sequence Length
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Enzymes Found
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Total Cut Sites
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Unique Cutters
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Visual Map

Cut Sites by Enzyme

What is Restriction Mapping?

Restriction mapping is a molecular biology technique that identifies and maps the locations where restriction enzymes cut DNA sequences. Restriction enzymes (endonucleases) recognize specific short DNA sequences and cleave the DNA at or near these recognition sites. This tool helps plan cloning strategies, verify constructs, and design diagnostic digests.

How to Use This Restriction Map Tool

Simple steps to map your sequence:

  1. Paste your DNA sequence (FASTA or plain text)
  2. View visual map showing enzyme positions
  3. Review statistics: enzymes found, cut sites, unique cutters
  4. Download the complete restriction map report

When to Use

Use restriction mapping when you need to:

  • Plan molecular cloning experiments and vector construction
  • Verify plasmid constructs and gene inserts
  • Design diagnostic restriction digests
  • Identify unique cutters for linearization
  • Check compatibility of restriction sites
  • Predict fragment sizes from enzyme digestion

Included Restriction Enzymes

This tool searches for 30+ common enzymes:

EcoRI, BamHI, HindIII, PstI, SacI,
SmaI, KpnI, XbaI, XhoI, SalI,
NotI, EcoRV, PvuII, NcoI, NdeI,
BglII, SpeI, ApaI, ClaI, HpaI,
and more...

Example Output

The tool provides:

  • Visual Map: Text-based representation showing enzyme positions along sequence
  • Statistics: Sequence length, number of enzymes, total sites, unique cutters
  • Site List: Complete list of all cut sites with positions
  • Downloadable Report: Full restriction map for lab notebook

FAQ

Q: What are unique cutters?
A: Enzymes that cut only once in your sequence. Essential for linearizing plasmids without creating multiple fragments.

Q: Does this tool check reverse complement?
A: Yes, the tool automatically searches both strands for palindromic enzyme recognition sites.

Q: Can I add custom enzymes?
A: Currently the tool includes 30+ common enzymes. Custom enzyme support may be added in future updates.