Reverse Complement DNA

Generate reverse complement, complement, or reverse of DNA sequences for primer design and sequence analysis.

What is Reverse Complement?

Reverse complement DNA tool generates the complementary strand in reverse orientation. Essential for primer design, PCR planning, and analyzing double-stranded DNA interactions.

How to Use This Tool

Quick guide to get results:

  1. Select operation: Reverse Complement, Complement, or Reverse
  2. Paste your DNA sequence
  3. Results appear instantly

When to Use

This tool is useful when you need to:

  • Designing PCR primers and probes
  • Finding palindromic sequences
  • Analyzing restriction enzyme sites
  • Converting between DNA strands

Example Input

Sample input:

GAATTCAAGCTTGAATTC

Try with your own sequence or use the Example button.

Example Output

Expected output format:

GAATTCAAGCTTGAATTC

Results can be copied or downloaded.

FAQ

Q: Does this tool handle FASTA format?
A: Yes, both single and multiple sequences.

Q: What's the difference between complement and reverse complement?
A: Complement replaces each base with its pair (A↔T, C↔G). Reverse complement does this AND reverses the sequence.