Reverse DNA/RNA Sequence

What is Reverse Sequence?

Reverse Sequence is a simple tool that reverses the orientation of DNA or RNA sequences from 5'-3' to 3'-5' direction. Unlike reverse complement, this tool only reverses the order of nucleotides without changing bases (A remains A, T remains T, etc.). This is useful for examining sequences in opposite orientation or preparing sequences for specific molecular biology applications.

How to Use This Reverse Sequence Tool

Quick guide to reverse your sequences:

1. Paste your DNA or RNA sequence (FASTA or plain text)
2. Results appear automatically showing reversed sequence
3. Download or copy the reversed output

When to Use

This tool is useful when you need to:

• View sequences in 3'-5' orientation for analysis
• Compare forward and reverse orientations
• Prepare sequences for specific cloning strategies
• Check sequence palindromes or patterns

Example Input

Sample input (5'-3' direction):

Try the Example button to see how it works.

Example Output

Expected output (3'-5' direction):

Note: Only the order is reversed, bases remain unchanged.

FAQ

Q: What's the difference between reverse and reverse complement?
A: Reverse only changes the order (5'-3' to 3'-5'), while reverse complement also changes bases (A↔T, G↔C).

Q: Does this tool handle FASTA format?
A: Yes, both FASTA headers and plain sequences are supported.

Q: Can I use this for RNA sequences?
A: Yes, works for both DNA and RNA sequences.