Serial Dilution Planner

What is Serial Dilution?

Serial dilution is a systematic method of reducing concentration by the same dilution factor in each step. It's essential for creating standard curves, determining optimal concentrations, and preparing samples for various assays.

How to Use This Planner

1. Enter your stock concentration and units
2. Choose dilution factor or concentration range method
3. Set the number of dilutions needed
4. Specify final volume per tube/well
5. Get step-by-step protocol with exact volumes

Common Applications

• ELISA standard curves
• qPCR/RT-PCR standards
• MIC (Minimum Inhibitory Concentration) assays
• Protein quantification standards
• Cell culture drug dilutions
• Antibody titration

Dilution Factor Guide

• 2-fold: Most common, good resolution
• 3-fold: Wider range, fewer points
• 5-fold: Quick screening
• 10-fold: Very wide range, log scale

Tips for Accuracy

• Mix thoroughly between dilutions
• Use fresh pipette tips for each transfer
• Pre-wet tips with solution before transfer
• Work quickly to minimize evaporation
• Label tubes clearly before starting

FAQ

Q: What's the difference between serial and parallel dilution?
A: Serial dilution uses each dilution as the source for the next, while parallel dilution makes all dilutions directly from the stock.

Q: How many dilutions should I make?
A: Typically 5-8 points cover a good range. Use more for wider concentration ranges or better curve fitting.