Six Frame Translation

Translate DNA sequences to protein in all six reading frames simultaneously

Translation Options

Highlight ORFs (M to *)
Show position numbers
Compact view (60 aa/line)

ORF Visualization

Reading Frames Detail

What is Six Frame Translation?

Six Frame Translation converts DNA sequences to protein in all possible reading frames - three on the forward strand and three on the reverse complement strand. This comprehensive translation helps identify all potential coding regions and is essential for gene prediction and sequence analysis.

How to Use This Six Frame Translation Tool

Get all six protein translations instantly:

  1. Paste your DNA sequence
  2. Select genetic code if non-standard
  3. View translations for all 6 frames
  4. ORFs highlighted from M to stop (*)

When to Use

This tool is essential for:

  • Finding all possible protein products
  • Gene prediction in unknown sequences
  • Identifying reading frame shifts
  • Analyzing both DNA strands
  • Detecting overlapping genes

Example Input

Sample DNA sequence:

ATGGCTAGCGATCGATCGATCGATCGATCGCTAGCTAGCTAGCTGACTGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCTAA

Contains start codon and stop codon.

Example Output

Six frame translations:

Frame +1: MASDRSSSSSLASSSLTDRSSSSSSS... Frame +2: WLARSSSSSSASSSHARSSSSSSSSS... Frame +3: GLAIDRSSSSQLLLQDRSSSSSSSSS... Frame -1: YSSSSSSSSSSSSSSSSSSSSSSSSS... Frame -2: IAIAIAIAIAIAIAIAIAIAIAIAIA... Frame -3: SRSRSRSRSRSRSRSRSRSRSRSRSR...

All six reading frames shown.

FAQ

Q: Why six frames?
A: DNA can be read in 3 frames on each strand (forward and reverse), giving 6 possible translations.

Q: What are the highlighted regions?
A: Regions from M (methionine) to * (stop) are potential open reading frames (ORFs).