Split Codons

What is Split Codons?

Split Codons divides coding DNA sequences into three separate sequences based on codon positions. This tool extracts nucleotides at positions 1, 2, and 3 of each codon for independent evolutionary and statistical analysis.

How to Use This Split Codons Tool

Quick guide to split your sequences:

1. Paste your coding DNA sequence (multiple of 3 nucleotides)
2. Results split automatically into 3 position sequences
3. Download or copy the separated sequences

When to Use

This tool is useful when you need to:

• Analyze codon positions separately for evolutionary studies
• Study synonymous vs non-synonymous substitution patterns
• Examine codon bias at different positions

Example Input

Sample coding sequence:

Try with your own sequence or use the Example button.

Example Output

Sequences split by position (FASTA format):

Each position extracted as separate FASTA entry.

FAQ

Q: Does this tool handle FASTA format?
A: Yes, both single and multiple FASTA sequences are supported.

Q: What if my sequence length is not divisible by 3?
A: The tool will process complete codons and note any incomplete trailing nucleotides.